Mach-1 V500css Mechanical Tester for 3D Surfaces Mapping in Indentation

Advantages and Current Limitations of Indentation

Contrary to other testing configurations in which a sample must be harvested, indentation is non-destructive and maintains the mechanical environment of the sample. This particularity of indentation thus allows the possibility to perform repeated testing at the same location and to test multiple sites with high spatial density, while leaving sample intact for subsequent characterizations. The present limitation of the indentation technique is due to the requirement that indentation be performed perpendicularly to the articular surface. Using a uniaxial mechanical tester for indentation, the researcher has to manually orient the sample surface perpendicular to the indentation axis at the desired location. This visual alignment creates a variation in the results and errors in the localization of the test.

Automated Indentation Mapping

With the use of the unique multiple-axis capabilities of the Mach-1 v500css tester, Biomomentum has developed innovative functionalities which allow all the advantages of indentation testing, while overcoming traditional limitations. This technological advancement is due to the addition of two functions to the Mach-1 Motion software: “Scan XY” and “Normal Indentation”. The “Scan XY” function moves the X and Y horizontal stages of the tester at predefined positions (contained in a list) prior to performing a series of functions at each of these positions. It should be mentioned that such a position list can be easily generated using a top view picture of the sample processed with Biomomentum’s “Cartilage Mapping” software. The “Normal Indentation” function precisely detects the height and orientation of the surface at the XY position and records the load while simultaneously moving the three stages of the tester at different speeds in order to move a spherical indenter with a predefined displacement profile along a virtual axis normal to the surface of the sample.

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Publications

2017

S. Sim, I. Hadjab, L.-A. Chevrolat, M. Masse, A.-L. Tong, P. Lavigne, M. Garon, E. Quenneville, and M.D. Buschmann. Combined Mechanical Characterizations Increases Sensitivity in the Assessment of Human Cartilage Degeneration. Orthopeadic Research Society Annual Meeting 2017 in San Diego, USA. Podium Presentation #252 on Tuesday Mar 21, 2017 at 3:25pm. Abstract

2016

S. Sim, A. Matuska, M. Garon, E. Quenneville, P. McFetridge and M.D. Buschmann. Cartilage Stiffness and Thickness Distributions Revealed by an Automated Indentation Technique in the Temporomandibular Joint. TMJ Bioengineering Conference, Barcelona, Spain, Sept 2016, Presentation #5032, Session Biomechanics Part I at 10:30AM on Day #2 (tentative time slot).

Sim S, Chevrier A, Garon M, Quenneville E, Lavigne P, Yaroshinsky A, Hoemann CD, Buschmann MD. Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage. J Orthop Res. 2016 Jun 9. doi: 10.1002/jor.23330. Abstract

S. Sim, A. Chartrand, A.-P. Lavallée, J. Tessier, M. Garon, E. Quenneville, and M.D. Buschmann. Development of a Sequence of Mechanical Tests for Articular Cartilage at a Single Location. Orthopaedic Research Society (ORS) Annual Meeting. Disney's Coronado Springs Resort, Orlando, Florida, USA, March 6-8, 2016. Poster #0527

2015

Hadjab I., Sim S., Chevrier A., Quenneville E., Garon M. and Buschmann M.D. Mechanical Assessment of Human Articular Cartilage Issued from Asymptomatic and Total Knee Replacement Donors. International Conference on the Mechanics of Biomaterials and Tissues (ICMoBT). Waikoloa, Hawaii, USA, December 6-10, 2015. Poster ICMOBT2015_0206.

Michael Seidenstucker. Ermittlung mechanischer Kennwerte mittels Indentation. Annual Meeting of the German Society for Biomaterials. Freiburg, Germany, November 12-14, 2015. Presentation MSB 05. Abstract / Presentation

Sim S., Garon M., Quenneville E. and Buschmann M.D. (2015) Wound Healing Revealed by a Novel Automated Indentation Technique. Biomedical Engineering Society (BMES) Annual Meeting, Tampa, FL, USA. Poster P-Th-596. Thursday, October 8, 2015, 9:30 AM - 5:00 PM.

Hadjab I., Sim S., E. Quenneville, M. Garon and M.D. Buschmann. (2015) Assessment of Human Articular Cartilage Issued from Asymptomatic and TKR Donors. Biomedical Engineering Society (BMES) Annual Meeting, Tampa, FL, USA. Presentation OP-Thurs-3-8. Thursday, October 8, 2015, 4:30 PM - 6:00 PM, Room 14.

Nickmanesh R., Stewart R., Snyder B., Grinstaff M., Wilson D. (2015) Contrast-Enhanced Computed Tomography (CECT) Reflects Stiffness of Intact Articular Cartilage. ISMRM Workshop on Imaging Based Measures of Osteoarthritis. Sept 11-14, 2015. Pacific Grove, CA, USA. Presentation on Sept 13 at 14:39. Abstract

Lavoie JF, Sim S, Quenneville E, Garon M, Moreau A, Buschmann MD and Aubin CE (2015) Mapping Articular Cartilage Biomechanical Properties of Normal and Osteoarthritic Mice Using Indentation. 21st Congress of the European Society of Biomechanics, Prague, Czech Republic, July 5-8, 2015, Podium O-295, Session: S62 - Animal biomechanics, Wednesday, July 8, 15:00 - 16:40. Abstract / Presentation

Sim S, Garon M, Quenneville E, and Buschmann MD (2015) Wound Healing Revealed by a Novel Automated Indentation Technique. Canadian Connective Tissue Conference, Laval University, Quebec City, Quebec, Canada, May 28-30, 2015. Podium Presentation. Session 1: Connective Tissue Remodeling I, Friday, May 29, 9:00 - 10:33. Abstract / Presentation

S. Sim, I. Hadjab, M. Garon, E. Quenneville, and M.D. Buschmann. Relevance of the Spatial Distribution Pattern of Mechanical Properties of Articular Cartilage in Animal Studies. International Cartilage Repair Society (ICRS) World Congress. Podium presentation 18.2.2 Osteochondral Grafts: Allografts and Synthetics, Sunday, May 10, 14:15 - 15:45, Sheraton 1-3. Abstract / Presentation

J.F. Lavoie, S. Sim, E. Quenneville, M. Garon, A. Moreau, M.D. Buschmann and C.-E. Aubin. Mapping Articular Cartilage Biomechanical Properties of Normal and Osteoarthritis Mice Using Indentation. International Cartilage Repair Society (ICRS) World Congress. Podium presentation 23.2.9 Osteoarthritis - Basic Science 2 and Biomechanics, Monday, May 11, 10:00 - 11:30, Chicago 9. Abstract / Presentation

S. Sim, I. Hadjab, M. Garon, E. Quenneville, M.B. Hurtig, M.D. Buschmann, and C.D. Hoemann. Evaluation of Entire Ovine Cartilage Repair Articular Surfaces: Mechanical and Electromechanical Assessment. International Cartilage Repair Society (ICRS) World Congress. Poster Presentation (P87). Abstract / Poster / ePoster

J.F. Lavoie, S. Sim, E. Quenneville, M. Garon, A. Moreau, M.D. Buschmann and C.-E. Aubin. Mapping Articular Cartilage Biomechanical Properties of Normal and Osteoarthritis Mice Using Indentation. OARSI World Congress on Osteoarthritis, April 30-3, 2015, Seattle, USA. Poster 391

S. Sim, I. Hadjab, M. Garon, E. Quenneville, and M.D. Buschmann. Relevance of the Spatial Distribution Pattern of Mechanical Properties of Articular Cartilage in Animal Studies. 61st Annual Meeting of the ORS, March 28-30, 2015, Las Vegas, USA. Poster 0359

2014

S. Sim, A. Chevrier, M. Garon, E. Quenneville, and M.D. Buschmann. Novel Technique to Map the Biomechanical Properties of Entire Articular Surfaces Using Indentation to Identify Degenerated (Osteoarthritis-like) Cartilage. 7th World Congress on Biomechanics, July 6-11, 2014, Boston, MA, USA. Poster 2229

Kolaczek S, Changoor A, Hurtig M, Gordon K, Getgood A (2014) Micro-CT visualization and indentation properties of whole meniscus following mercury exposure. Annual Meeting of the ORS, March 15-18, New Orleans, Louisiana, USA. Abstract / Poster